Other

Part:BBa_K648104:Design

Designed by: Alex Bina   Group: iGEM11_Penn_State   (2011-10-23)


RecA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needed to remove the restriction sites from the genomic sequence in order to put RecAI into BioBrick plasmid.


Source

The RecAI gene comes from the genome of the DH10B Escherichia coli bacteria.

References